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Demonstration of the role of the DnaK chaperone system in assembly of 30S ribosomal subunits using a purified in vitro system

¹ Iowa State University, Department of Biochemistry, Biophysics, and Molecular Biology, Ames, Iowa 50011, USA
² Johns Hopkins University School of Medicine, Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Baltimore, Maryland 21205, USA

Reprint requests to: Gloria M. Culver, Iowa State University, 4216 Molecular Biology Building, Department of Biochemistry, Biophysics and Molecular Biology, Ames, IA 50011, USA; e-mail: gculver{at}iastate.edu.

Recently, there has been controversy regarding the ability of the DnaK chaperone system to facilitate Escherichia coli 30S subunit assembly at otherwise nonpermissive conditions. Here, we present additional data indicating that purified DnaK chaperone assembled 30S subunits are functional. Additionally, explanations for reported differences are discussed.

Keywords: DnaK chaperone system; 16S rRNA; ribosome assembly

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